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  5. CEP® X/Y DNA Probe Kit

CEP® X/Y DNA Probe Kit 〈研究用試薬〉

7J20-50 20 Assays (without control slides)

The CEP® X/Y DNA Probe Kit may be used as an adjunct to standard karotyping to evaluate engraftment success in recipients of sex mismatched bone-marrow transplantation by determining the proportion of XX and XY donor cells. Following transplantation, an assessment of the proportion of cells belonging to the donor and to the recipient can be used to evaluate engraftment, detect the presence of clonal neoplasms and determine disease recurrence. This probe kit offers a limit of detection of 1% through a combination of CEP® X and CEP® Y fluorescently labeled DNA probes for specific regions of chromosome X and chromosome Y, respectively. This probe provides rapid (results in 3 hours or less) and accurate identification of the genetic sex of the bone marrow cells. Bone-marrow transplantation is a critical therapeutic strategy in the management of hematologic malignancies, such as:

  • Chronic Myelogenous Leukemia (CML)
  • Acute Myeloid Leukemia (AML)
  • Acute Lymphocytic Leukemia (ALL)
  • Myeloproliferative Disorder (MPD)
  • Chronic Lymphocytic Leukemia (CLL)
  • Myelodysplastic Syndrome (MDS)
  • Other hematologic disorders not otherwise specified (including hyper-proliferative states such as polycythemia vera, leukemoid reaction, and lymphoproliferative disorders)

DNA Probe Description

The CEP® X/Y probe is a mixture of a SpectrumOrange labeled CEP® X probe and a SpectrumGreen labeled CEP® Y probe specific for the alpha satellite centromeric region of chromosome X and the satellite Ⅲ (Yq12) region of chromosome Y.

Results of Hybridization

In a normal male cell, the expected pattern for a nucleus hybridized with the CEP® X/Y DNA Probe is the one orange, one green (1O1G) signal pattern. In a normal female cell the two orange (2O) single pattern for female donor cells will be observed.

Materials Provided With the CEP® X/Y DNA Probe Kit:

  • CEP® X/Y DNA probe pre-denatured in hybridization buffer (220μL)
  • NP-40 (detergent for wash solution 1000μL)
  • DAPI II counterstain (300μL)
  • 20X SSC (66g)

Control slides for the CEP® X/Y kit are also sold separately. See Order No. 7J21-11, Order No. 7J21-12.

Ideogram

Probe Image

Female

Male

Bibliographies

  1. Byrd, J., et al. Clin. Cancer Res. 4 (1998): 1235-41.
  2. Escudier, S., et al. Blood 81 (1993): 2702-7.
  3. Heim, S., et al. Cancer Cytogenetics 2nd ed. (1995): 377.
  4. Jenkins, R., et al. Blood 79 (1992): 3307-15.
  5. Najfeld,V., et al. Bone Marrow Trans. 19 (1997): 829-34.